Peripheral nerve injuries are frequently encountered in veterinary clinical practice and experimental research, especially in cases of trauma, orthopedic surgeries, bite wounds, and iatrogenic injuries. Accurate evaluation of peripheral nerve damage and subsequent regeneration is essential for diagnosis, prognosis, selection of therapeutic strategies, and assessment of treatment outcomes. Unlike gross injuries that are easily visible, peripheral nerve dysfunction often requires a combination of clinical, functional, electrophysiological, histological, and behavioral assessment techniques. Over the years, numerous methods have been developed to evaluate axonal degeneration, regeneration, target organ reinnervation, and functional recovery in animals. This article presents a comprehensive and simplified overview of commonly used evaluation methods for peripheral nerve injury in animals, including axonal regeneration tests, nerve conduction studies, target reinnervation assessment, sensory and motor function tests, muscle evaluation techniques, gait analysis, and advanced electrophysiological and behavioral assessments.

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